Figure 3

Upregulation of MMP-12 gene in post-OVA in KO lung. Real time PCR analysis was used to quantitate expression of mRNA for the particular genes as calculated by relative index of Ct values normalized to GAPDH by real time PCR. PCR was carried out using the comparative Ct method (Applied Biosystems software) with SYBR Green PCRcore reagents (Applied Biosystems) and anlysed using Applied Biosystems 7900HT Real-Time PCR System software SDS 2.2.1. All primers used were specific to mouse. * denotes p value<0.01 compared to WT+OVA values. # denotes p value<0.01 compared to WT+alum (control baseline values). n=5/group pooled from 2 experiments. Expression of the gene of interest was expressed in relative values normalized to the values obtained for mouse GAPDH. There was 10-fold increase in the gene expression of MMP-12 in OVA-treated WT vs. control WT whereas saline-treated NOX KO lungs showed 6.5-fold increase over untreated control mouse lung. However, post-OVA treatment, NOX KO mouse lung showed MMP-12 shooting up 4.7-fold over untreated KO. # denotes p value <0.01 compared to control untreated, * denotes p value <0.01 compares untreated KO versus OVA-treated KO lung.