Table 1 List of mouse primers for real time PCR
From: Role of T cells in a gp91phox knockout murine model of acute allergic asthma
Cell marker | Gene | Forward primer | Reverse primer |
|---|---|---|---|
House- keeping genes | GAPDH | CGTCCCGTAGACAAAATGGT | TCAATGAAGGGGTCGTTGAT |
β-actin | GTGGGCCGCTCTAGGCACCAA | CTCTTTGATGTCACGCACGATTTC | |
Cytokine genes | IFN-γ | GCGTCATTGAATCACACCTG | TGAGCTCATTGAATGCTTGG |
IL-1α | TCAAGATGGCCAAAGTTCCT | TGCAAGTCTCATGAAGTGAGC | |
IL-1β | TGAAGCAGCTATGGCAACTG | GGGTCCGTCAACTTCAAAGA | |
IL-2 | AACCTGAAACTCCCCAGGAT | CGCAGAGGTCCAAGTTCATC | |
IL-3 | CCGTTTAACCAGAACGTTGAA | CCACGAATTTGGACAGGTTT | |
IL-4 | GGCATTTTGAACGAGGTCAC | AAATATGCGAAGCACCTTGG | |
IL-5 | ATGGAGATTCCCATGAGCAC | AGCCCCTGAAAGATTTCTCC | |
IL-6 | AACGATGATGCACTTGCAGA | GGTACTCCAGAAGACCAGAGGA | |
IL-10 | TGAATTCCCTGGGTGAGAAG | TGGCCTTGTAGACACCTTGG | |
IL-12β | ATCGTTTTGCTGGTGTCTCC | CATCTTCTTCAGGCGTGTCA | |
IL-13 | CCTCTGACCCTTAAGGAGCTT | ATGTTGGTCAGGGAATCCAG | |
MCP-3 | TCTGTGCCTGCTGCTCATAG | CTTTGGAGTTGGGGTTTTCA | |
Growth factor genes | TGFβ2 | GGAGGTTTATAAAATCGACATGC | GGCATATGTAGAGGTGCCATC |
VEGFa | TACCTCCACCATGCCAAG | TGGTAGACATCCATGAACTTGA | |
VEGFb | GGCTTAGAGCTCAACCCAGA | TGGAAAGCAGCTTGTCACTTT | |
VEGFc | GGGAAGAAGTTCCACCATCA | TCGCACACGGTCTTCTGTAA |