Table 1 Oral food challenge and Rush OIT protocol
Oral food challenge | |
|---|---|
Procedure | Open food challenge |
Materials | Fresh CM or MH-CM (heated for 100 s in 550 W microwave oven) or |
Initial dose | 0.1 mL 1 mL (if no anaphylaxis is suspected) |
Subsequent dose | 0.2, 0.5, 1,2, 5. 10, 20 and 40 mL 2, 5, 10, 20 and 40 mL (if no anaphylaxis is suspected) |
Interval | Every 30 min |
Threshold dose | Defined as the last dose in OFC at which only objective symptoms occurred and the levels of severity of allergy to CM [20] |
Rush OIT | |
Initial dose | Starting approximately at a tenth of the threshold dose determined at OFC using MH-CM 1.2-fold for each patient |
Increase in dose | Starting with MH-CM, the dose was increased approximately 1.2-fold every time Ingest MH-CM 2–4 times a day at 2-h intervals If gradea 2 or 3 develops, the same dose or the previous tolerated dose was repeated If gradea 4 develops, OIT was stopped and the previous tolerated dose was repeated on the next day When reaching 200 mL of MH-CH, the ingestion was once a day No further increases in dosage because of repeated adverse events, after the highest tolerated dose was continued for 3 consecutive days without any allergic reaction, the rush OIT was terminated |
Maintenance | Ingest the maintenance dose of 200 mL of MH-CM everyday for maintenance at home If the subject did not reach the target dose of 200 mL of MH-CM, the loading dose was gradually increased by 1 mL per day until the target dose of 200 mL was reached and the dose was continued |
MH-CM to fresh CM | In cases where no adverse reactions were observed for 2 months during a daily intake of 200 mL of MH-CM, the time spent heating the CM in the microwave oven was gradually shortened by 10 s every week |