Fig. 1
From: Human placental extract suppresses mast cell activation and induces mast cell apoptosis
Human placental extract (HPE) inhibits proliferation and induces apoptosis of mast cells. A C57 or HMC-1 cells were treated with human placental extract (HPE) for 4, 8 or 16 h followed by incubation with CCK-8. Color development was analyzed by microplate reader and cell proliferation is shown as O.D values. B, C 2.5 × 105 of C57 or HMC-1 cells were incubated in the absence or presence of HPE at various concentrations as indicated for 4, 8 or16 h, following with Ki-67 detection by flow cytometric analysis. The flow cytometric histograms and mean fluorescence intensity (MFI) was shown in B. Or cell apoptosis was analyzed by flow cytometric analysis in C. Data are shown as mean ± SEM (n = 3) of three separate experiments. *P < 0.05, **P < 0.01, ***P < 0.001, using the one-way ANOVA with Tukey’s multiple comparison test for statistical significance