Fig. 3

Human placental extract (HPE) suppresses activation of mast cell following treatment with C48/80 or IgE/anti-IgE. C57 or HMC-1 cells were pre-treated with or without HPE (30 ng/mL) for 4, 8 or 16 h followed by washing. Cells were next subjected to treatment with anti-DNP IgE or activation through C48/80 as indicated. A β-hexosaminidase (β-hex) was measured in culture supernatant with anti-DNP IgE after pre-treatment by HPE for 4, 8 h. B β-hexosaminidase (β-hex) was measured in culture supernatant with C48/80 after pre-treatment by HPE for 4, 8 h. C Cytokines of IL-4, IL-6, IL-13 or TNF-α were analyzed in culture supernatant using cytometric bead array analysis after treatment with C48/80 for 4, 8 or 16 h. Heatmap of cytokine release was shown. Data are shown as mean ± SEM (n = 3) of three separate experiments.*P < 0.05, **P < 0.01, ***P < 0.001, using the two-way ANOVA with Tukey’s multiple comparison test for statistical significance