Fig. 4

Antigen phagocytosis by mast cells is not affected by human placental extracts (HPE). C57 or HMC-1 cells were treated with or without HPE at concentrations and durations as indicated followed by stimulation with 80 mg/mL of OVA-AF488 for 2 h. OVA uptake was assessed by flow cytometry (A) or a confocal microscope (B). The fluorescence intensity of engulfed OVA was pooled in (C). Data in (C) are presented as mean ± SEM (n = 3) of three separate experiments. Using the one-way ANOVA with Tukey’s multiple comparison test for statistical significance