Fig. 7
From: Human placental extract suppresses mast cell activation and induces mast cell apoptosis
Human placental extract (HPE) suppresses histamine release in cutaneous anaphylaxis reaction. The PCA was induced in BALB/c mouse pre-treatment with HPE for 4 (A), 8 (B) or 16 h (C) as described in Fig. 6, and followed by peroxidase-based immunostaining using an anti-histamine antibody. The relative histamine expression was analysis with Image J software. The typical views were shown (left panels) and the relative histamine expression was quantified and plotted (right panels). At least five randomly chosen areas were scanned for determining the intensity of histamine which was expressed as optical density (OD) per area. Data are shown as mean ± SEM (n = 5) of three separate experiments. *** P < 0.001, using the unpaired Student t test for statistical significance