Fig. 6

Circ_0070934 had a targeted binding relationship with miR-199a-5p. A There are potential base binding sites between circ_0070934 and miR-199a-5p. B Dual-luciferase reporter assay was conducted to explicit the binding relationship between circ_0070934 and miR-199a-5p. C MiR-199a-5p-pull-down and NC-pull-down were the two groups of cells transfected with miR-199a-5p and NC probes, respectively. The input was the sample control, equivalent to total RNA, and the pulldown was the RNA after magnetic bead pull-down. There are four groups of RNA. The expression of hsa circ 0070934 and GAPDH were detected by qPCR. The data were presented as mean ± SEM. Our experiments were repeated three times. ns, no significant; P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001