Figure 3

Effects of testing samples on pathological changes in the lungs. No pathologic alterations were seen in the lungs of the control (A). Mild proliferation of goblet cells that have mucus stained pink with PAS in airway epithelium and moderate fibrous thickening of the subepithelial layer in the airway exposed to B.ad 8 alone (B). Very slight proliferation of bronchial cells exposed to H-ASD alone (C). Mild proliferation of goblet cells that have mucus stained pink with PAS in the airway epithelium and infiltration of inflammatory cells in the submucosa of airway and mild fibrous thickening of the subepithelial layer in the airway exposed to H-ASD + B.ad 8 (D). Very slight proliferation of goblet cells in the airway epithelium and slight infiltration of inflammatory cells in the submucosa of airway exposed to OVA alone (E). Mild to moderate proliferation of goblet cells in the airway epithelium and infiltration of inflammatory cells in the submucosa of airway along with mild fibrous thickening of the subepithelial layer in the airway exposed to OVA + B.ad 8 (F). Slight goblet cell proliferation and slight infiltration of inflammatory cells in the submucosa of airway exposed to OVA + H-ASD (G). Moderate goblet cell proliferation, numerous inflammatory cells in the submucosa of airway, and serious fibrous thickening of the subepithelial layer in the airway and macrophages scattered in alveoli exposed to OVA + H-ASD + B.ad 8 (H). (A–H) PAS stain; bar = 100 μm.