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Table 2 Effect of cryopreservation on Treg numbers and phenotype

From: Patients with posttraumatic stress disorder exhibit an altered phenotype of regulatory T cells

 

Fresh PBMCs (N = 5)

Frozen PBMCs (N = 5)

p value

CD3+CD4+

51,4

51,38

0,99

Tregs (CD3+CD4+CD25+FOXP3+CD127lo/-)

2,16

2,15

0,96

CD127loHLA-DR- Tregs

35,8

34,8

0,77

CD127loHLA-DR+ Tregs

35,96

37,56

0,53

CD127-HLA-DR+ Tregs

20,54

20,8

0,91

CD127-HLA-DR- Tregs

7,67

6,77

0,36

  1. The impact of the cryopreservation method was tested by measuring Treg frequencies and phenotype in fresh and freeze/thawed PBMCs of a small random sample (N = 5). Treg frequencies and phenotype of the same subjects before and after cryopreservation were compared with paired samples T test (data represented as group means). The results indicate no effect of cryopreservation on Treg numbers and phenotype.
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Allergy, Asthma & Clinical Immunology

ISSN: 1710-1492

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