Volume 10 Supplement 1
Skin prick tests to multiple pollens and prevalence of IgE specific to profilin
© Plunkett et al; licensee BioMed Central Ltd. 2014
Published: 3 March 2014
Panallergens like profilin are proteins that have very similar sequences and structure. People can develop IgE specific to these highly cross reactive panallergens. The purpose of this study was to determine the prevalence of profilin sensitivity and the contribution to multiple allergen skin test responses. Sensitivity to profilin was established by IgE binding to purified profilins. Possible effects on skin test results were studied by including Queen Palm (QP) allergen in the skin prick testing panel as a possible source of profilin. QP is a species with a low probability of pollen exposure.
subjects from Central Texas and Northern California were recruited for SPT with Multi-Test®II to a panel of up to 51 allergens. Several extracts were tested for profilin and spanned a range of 0 – 14 microgram/mL. QP containing 10ug/mL profilin was added to the panel as a possible source of panallergen. IgE to various purified component major allergens and profilin was determined using Thermo Fisher ISAC microarray or ADVIA Centaur.
of 65 subjects (35%) had a positive IgE to at least one profilin. Of these, 9 had a >3mm wheal to QP. 20 of 65 subjects (32%), had a positive QP SPT, a similar prevalence of positive SPT as either Timothy grass (43%) or birch (34%) pollen. Subjects reacting by SPT to QP also reacted to multiple allergens, 21 – 100% of allergens tested (average 58%). The multiple allergen SPT sensitivity (percent of 16 allergens) correlated with in vitro profilin IgE binding, QP SPT+, sIgE+, 75%; QP SPT+, sIgE-, 56%;QP SPT-, sIgE+, 40%; QP SPT-, sIgE-, 32%.
Prevalence of IgE to profilin in the current study is similar to reports from European studies. The association of IgE specific to profilin and Queen Palm pollen skin responses suggests this low probability allergen may be useful to identify panallergen responses responsible for multiple sensitivities.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.