Volume 10 Supplement 2

Canadian Society of Allergy and Clinical Immunology and AllerGen Abstracts 2014

Open Access

Clinical evaluation of an allergen Challenge TheatreTM

  • Jacob Karsh1,
  • Suzanne Kelly1,
  • Jimmy Yang1,
  • Rob Perrins1 and
  • William H Yang1
Allergy, Asthma & Clinical Immunology201410(Suppl 2):A21

https://doi.org/10.1186/1710-1492-10-S2-A21

Published: 18 December 2014

Background

Allergen challenge chambers expose allergen-sensitive subjects to a predetermined concentration of allergen in a closed, controlled environment and provide a mechanism to induce clinical symptoms and measure the effect of medication.

Methods

A preliminary evaluation of the capabilities of the newly constructed Red Maple Trials Allergen Challenge TheatreTM was performed. Health Canada and a provincial Ethics Board approved the study. After signing informed consent, patients with a history of grass allergy, not on allergy medications and with a positive skin prick test to grass antigen (≥ 3 mm) were exposed for 3 hours to timothy grass pollen (Phleum pratense) in the allergen challenge theatre. Total nasal (TNSS) and rhinoconjunctivitis symptom scores (TRSS) were recorded at baseline and every 30 minutes during the challenge.

Results

32/50 patients evaluated demonstrated a positive skin prick test and were challenged. Baseline TNSS and TRSS (Mean± SD) were 0.6±1.04 and 0.6±1.07 respectively. Symptom scores reached a plateau at 30 minutes (TNSS 4.8±2.68; TRSS 5.8±3.69) and remained steady for the 180-minute exposure period reaching final values of TNSS 3.7±2.16; and TRSS 5.8±3.79. Because entry to a therapeutic trial usually requires achieving a TNSS ±5 during a priming exposure, we calculated the results for the 17/32 patients reaching this score at 30 minutes (TNSS 6.65±2.21; TRSS 8.35±3.18). Scores held steady and at 180 minutes were: TNSS 4.71±1.69; TRSS 7.88±3.06. No unexpected adverse events were reported during the challenge.

Conclusions

The Red Maple Trials allergen exposure theatre demonstrated the capacity to induce symptoms of appropriate intensity upon allergen challenge. The chamber with a seating capacity of 99 places has the ability to evaluate large test groups at a time.

Authors’ Affiliations

(1)
Red Maple Trials Inc

Copyright

© Karsh et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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