Figure 4
Expression of Bim induced by FcεRI and FcγRIIB or by FcεRI alone. C57 mast cells were activated as in Figure 1B for 24 hours. Cells sensitized only with 0.1 μg/mL of anti-dinitrophenyl immunoglobulin E (lgE) and incubated with or without 5 μg/mL of rabbit anti-mouse CD16/CD32 (FcγIII/II receptor) monoclonal antibody (2.4G2 rat Ab) were used as controls. Cell lysates were prepared, and the induction of Bim was analyzed by Western blot with the indicated antibodies. The result is representative of three independent experiments. MAR = mouse anti-rat; TNP = trinitrophenyl.