Figure 3

Decreased IL-10 production and suppressive function of allergic asthmatic pulmonary Treg. A. (Top) IL-10 expression by PMA/I activated pulmonary Treg from AA, HC, and NA subjects (n = 13). Data represented intracellular flow cytometric and ELISA results. (Bottom) Representative FACS plots of IL-10 expression by pulmonary Treg from different subject groups. B. Suppressive activity of pulmonary Treg against autologous Teff from AA, HC, and NA subjects represented as thymidine counts in suppression assays (n = 13). C. Allogeneic suppression assays with pulmonary T cells from AA and HC subjects (n = 7). Treg and Teff were cultured at 1:1 and 1:4 ratios. Kruskal Wallis tests (A, B) and Wilcoxon tests (C) were used for statistical analysis. Horizontal bars represented median values as indicated throughout the figure.