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Volume 6 Supplement 2

Canadian Society of Allergy and Clinical Immunology Annual Scientific Meeting 2010

Prolonged elevation of serum tryptase resulting from intraoperative anaphylaxis to methylene blue

Allergy, Asthma & Clinical Immunology volume 6, Article number: P29 (2010) Cite this article

Background

Intraoperative anaphylaxis during sentinel lymph node biopsy is a well known phenomenon occurring with administration of patent blue and isosulfan blue dyes, with an incidence of 2.2% and 1.1%, respectively (1,2). Methylene blue has been reported as being a safer alternative with only a few case reports demonstrating anaphylaxis and large studies demonstrating no incidence of anaphylaxis in 224 patients (3,2).

Case report

A 62 year old female undergoing sentinel lymph node biopsy and right lumpectomy was administered methylene blue alongside anaesthetics. Two minutes after administration of methylene blue, the patient entered anaphylactic shock with hypotension, discolouration, and angioedema. Fluid, steroids, and vasopressors were administered and the patient stabilized, at which point the operation continued. Serum taken an hour after anaphylaxis demonstrated a tryptase of 34 ng/ml. A sample six hours later demonstrated prolonged elevation of tryptase at 58 ng/ml. A baseline sample was within normal limits (<12 ng/ml) at 3 ng/ml. Skin prick and intradermal testing was negative for penicillin, ancef, latex, rocuronium, and propofol. Intradermal testing, however, was positive for methylene blue at 1/10, although a non-irritating concentration has yet to be established.

Discussion

Serum tryptase peaks at one to two hours post anaphylaxis with a subsequent half-life of two hours (4). We demonstrate tryptase levels that increase with time, rather than decrease, subsequent to exposure to methylene blue. Serum tryptase levels in response to methylene blue anaphylaxis have not been reported previously.

Conclusion

Methylene blue can cause anaphylaxis with a prolonged elevation of serum tryptase levels.

References

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Author information

Affiliations

  1. University of Manitoba, Winnipeg, Manitoba, Canada, R3E 3P5

    Sacha Oomah, Allan Becker & Chrystyna Kalicinsky

  2. Diagnostic Services of Manitoba, Winnipeg, Manitoba, Canada, R2H 2A6

    Tom Dembinski

  3. Allergy and Clinical Immunology, Health Sciences Center, Winnipeg, Manitoba, R3A 1R9

    Allan Becker & Chrystyna Kalicinsky

Authors
  1. Sacha Oomah
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  2. Tom Dembinski
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  3. Allan Becker
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  4. Chrystyna Kalicinsky
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Corresponding author

Correspondence to Chrystyna Kalicinsky.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Oomah, S., Dembinski, T., Becker, A. et al. Prolonged elevation of serum tryptase resulting from intraoperative anaphylaxis to methylene blue. All Asth Clin Immun 6, P29 (2010). https://doi.org/10.1186/1710-1492-6-S2-P29

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Keywords

  • Methylene Blue
  • Sentinel Lymph Node Biopsy
  • Angioedema
  • Rocuronium
  • Tryptase

Allergy, Asthma & Clinical Immunology

ISSN: 1710-1492

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