Figure 1

Schematic overview of CMP sensitization and challenge protocol in Balb/C mice. (a) Intragastrically sensitized (gavage) group. Mice were sub-divided into 5 treatments and sensitized intragastrically for 4 weeks as follows (n = 6); CTL-: PBS; CTB: PBS+ CTB (0.25 μg/g BW); CTL+: CMP (1 mg/g BW) + CTB; LGG1: CTL+ mice supplemented with LGG for 5 weeks and LGG2: CTL+ mice supplemented with LGG for 4 weeks. At d 35, all sensitized mice were intragastrically challenged two times with CMP (30 mg/mouse) at 30 min apart. Two hours after final challenge, mice were euthanized with CO2 inhalation and blood and spleen samples were collected. (b) Intraperitoneally sensitized (IP) groups. Mice were sub-divided into 4 treatments and sensitized intraperitoneally for 2 weeks as followed (n = 6); CTL-: PBS; CTL+: CMP (10 mg/mouse); LGG1: CTL+ mice supplemented with LGG for 5 weeks and LGG2: CTL+ mice supplemented with LGG for 4 weeks. At d 35, all sensitized mice were intragastrically challenged two times with CMP (30 mg/mouse) at 30 min apart. Two hours after final challenge, mice were euthanized with CO2 inhalation and blood and spleen samples were collected.