Figure 2
From: Allergen-specific T cell quantity in blood is higher in allergic compared to nonallergic individuals
Example of calculation of the index of the quantity of allergen-specific Th cells and the number of precursor cells of acquired allergen-specific cells (needed to calculate the absolute count of allergen specific Th cells). The percentage of the CFSElow cells after the 7 day culture is referred to as the "index" of the quantity of allergen-specific cells (in this example, Timothy-specific Th index). The number of precursor cells of acquired allergen-specific cells (in this example, precursor cells of CFSElow Th cells), determined using the ModFit software, and was used to estimate the absolute count of allergen-specific Th cells. Only generations 3 and higher were considered as allergen-specific cells and generations 0, 1 and 2 were omitted from the calculation.