Testing an emerging animal model for use in the allergenicity assessment of food
Allergy, Asthma & Clinical Immunology volume 7, Article number: A1 (2011)
The regulatory assessment of novel food includes tests for allergy. The World Health Organization suggests tests in an animal model of allergy despite the lack of a validated model. We aimed to confirm if C3H/HeJ mice would respond to food of high allergenic potential (peanut), but not to food of low allergenic potential (turkey, potato, spinach).
In the first study, C3H/HeJ mice were orally treated, once per week for two weeks, with adjuvant and 0 or 2 mg of peanut or turkey. A second study used adjuvant and 0, 0.1, 1 or 2 mg of peanut, potato or spinach. Blood IgE antibodies and spleen interleukin-4 were quantified.
Mice treated with 2 mg peanut developed peanut-specific IgE levels which were significantly higher than control mice (p<0.001, n=10/group). Mice treated with 2 mg turkey developed a similar IgE response to turkey (p<0.001, n=10/group). In the second study, allergy was only triggered in one of ten mice treated with 2 mg peanut. Two of ten mice exposed to 1 mg potato had a response. There were no IgE responders to spinach. Spleen cells from both the peanut- and the spinach-treated mice secreted more allergy-promoting interleukin-4 than controls (p<0.01, n=7-24/group). Levels were not modified in potato-treated mice.
C3H/HeJ mice developed food allergy markers to peanut. However, the incidence varied between experiments. Some mice developed a similar response to foods with low allergenic potential. Thus, this model may not be appropriate for safety assessment of novel food.
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Lefebvre, D.E., Ross, N., Coady, L. et al. Testing an emerging animal model for use in the allergenicity assessment of food. All Asth Clin Immun 7 (Suppl 2), A1 (2011). https://doi.org/10.1186/1710-1492-7-S2-A1
- Animal Model
- World Health Organization
- Control Mouse
- Food Allergy
- Spleen Cell