Skip to main content
  • Meeting abstract
  • Open access
  • Published:

Assessment of the immune-modulatory activity of sialylated fraction of IVIg in a murine model of allergic asthma


Intravenous immunoglobulin (IVIg) has potent immune-modulating properties. In OVA-challenged mice, we demonstrated that IVIg markedly attenuates airway hyperresponsiveness (AHR) and abrogates airways inflammation, accompanied by substantial induction of antigen-specific Foxp3+ Treg from non-Treg precursors.


Mice were sensitized (i.n.) with OVA and then received IVIg or sialic acid enriched IVIg (SA-IVIg) fragments (i.p.), and then underwent challenge (i.n.). The induction of CD4+CD25+Foxp3+Treg was determined by flow-cytometry. AHR was measured, using a flexiVent small animal ventilator. Phenotypic properties of dendritic cells (DC) from various experimental groups were assessed by flow-cytometry. Expression of DCIR on DC was evaluated by flowcytometry and ICC. Adoptive transfer of DC was carried out to show the tolerogenic activity of IVIg-primed DC.


IVIg and the SA-IVIg fraction induced Treg and abrogated AHR in OVA-challenged mice comparably. It followed by tolerogenic predisposition of DC (decrease of CD80/CD86 expression and IFN-γ production and increased level of IL-10). Adoptive transfer of DC from IVIg treated mice to OVA-challenged WT syngeneic mice has the similar anti-inflammatory activity of IVIg/SA-IVIg. Expression of DCIR (Inhibitory C-type lectin receptors) on DC of IVIg and SA-IVIg treated mice increased significantly.


IVIg induces Treg likely via conferring tolerogenic activities to DC. This mechanism is dependent on sialylated fraction of IVIg. DCIR is an inhibitory C-type lectin receptor that can be targeted by SA-IVIg and induce an inhibitory signal into the ligated cells. More dissection is required to confirming the role of DCIR in this model.

Author information

Authors and Affiliations


Rights and permissions

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Massoud, A.H., Ablona, A., Mourad, W. et al. Assessment of the immune-modulatory activity of sialylated fraction of IVIg in a murine model of allergic asthma. All Asth Clin Immun 7 (Suppl 2), A29 (2011).

Download citation

  • Published:

  • DOI: