Figure 1

IL-7Rα and L-selectin, are required for murine peanut-induced anaphylaxis. Mice were initially sensitized using peanut antigen and cholera toxin via oral gavage for 4 consecutive weeks. Two weeks following the final sensitization, mice were challenged i.p. with crude peanut extract. Body temperature (A, D) and average observed clinical scores (B, E) monitored every 10 minutes for 40 minutes post-injection. Following the 40-minute endpoint, blood levels of histamine were assayed (C, F). Control mice were challenged with peanut immediately before monitoring. (IL7R^−/− and Ly5.1 n = 3, representative of 4 experiments; L-Sel^−/−n = 8, Bl/6 n = 7, and control mice n = 3, representative of 5 experiments; *represents p < 0.05; **represents p < 0.01; Error bars = SEM).