Fig. 4

Changes in CD8 memory cell subsets during pollen season. Among CD8 cells the following memory cell subsets were separated: naïve (CD45RA+ CCR7+), central memory (CD45RA− CCR7+), effector memory (CD45 RA− CCR7−), CD45RA+ effector (CD45RA+ CCR7−). Cytometric example (a) and changes in percentages of the subsets (b) are shown. V1—before allergy season, V2—during allergy season immediately following the appearance of symptoms, V3—2 weeks after onset of symptoms, CTRL—control group; data are shown as mean ± SEM; *P < 0.05 in paired samples t test; ^$ P < 0.05 in repeated measures ANOVA, testing all three patients’ visits. Comparisons of cell populations for pairs of single patients’ visits were performed using paired samples t test, while comparisons of cell populations between single patients visit and control subjects were performed using unpaired samples t test