Fig. 6

Expression of CXCL10 stimulated by TNF, IL-1β and IFN-γ and inhibition effect by pre-incubated ITLN-1. 30 min pre-incubated by ITLN-1 inhibited expression of CXCL10 mRNA (a) and CXCL10 protein (b) in HFL-1 stimulated by cytomix (TNF, IL-1β and IFN-γ, 10 ng/mL each). a ITLN-1 at a concentration of 500 ng/mL inhibited cytomix induced CXCL10 mRNA (p = 0.0136). b ITLN-1 at a concentration of 250 and 500 ng/mL inhibited cytomix induced CXCL10 protein (p < 0.0001 and p = 0.0113, respectively). c Phospho (p)-STAT1/total (t)-STAT1 level stimulated by cytomix and pre-incubated ITLN-1 in HFL-1. Phospho (p)-STAT1/t-STAT1 level was increased at 5 and 15 min, 30 min pre-treated ITLN-1 was signify inhibited phosphorylation of STAT-1 (p = 0.0006 at 15 min and p = 0.0063 at 5 min)