Fig. 1

Identification of ILC2s from human peripheral blood using multi FACS staining. Lymphocytes were identified from whole PBMCs (left) and Lineage-negative cells gated (middle). Lineage-negative cells were further assessed for expression of CD127 and CRTH2 (CD294) or isotype control staining (right). ILC2s were identified as Lineage-negative CD127⁺CRTH2⁺ lymphoid like cells. Example shown is an asthmatic patient with pollen sensitization. FSC forward scatter. SSC side scatter