Fig. 2

Effect of Rgs2 deficiency on LPS-induced cell influx into bronchoalveolar lavage. Wild type mice were exposed to aerosolized PBS (20 ml) or LPS from E. coli 0127:B8 (20 ml of 150 μg/ml) for 30 min and bronchoalveolar lavage (BAL) was performed after 3, 6 or 24 h of LPS exposure. a Total cell counting in BAL fluid was performed. Data (N = 4–6) are plotted as mean ± SE. Significance compared against PBS control was assessed by ANOVA with a Dunnett’s post test. *P < 0.05, ***P < 0.001. b Following DiffQuick staining of cytospin slides, differential cell counting was performed. Data (N = 4–6) are plotted as mean ± SE. Significance compared with PBS control was assessed by ANOVA with a Dunn’s post test. *P < 0.05, **P < 0.01. c Wild type (WT) and Rgs2^−/− (KO) mice were exposed to aerosolized LPS, as above. The BAL fluid was collected after 3 and 24 of LPS treatment for counting of total cell numbers and the data (N = 6–7) plotted as mean ± SE. d After Diff-Quick staining of cytospin slides, differential cell counting was performed and the data (N = 4–7) plotted as mean ± SE. In C and D, significance was tested between WT and KO using Mann–Whitney U test. No significant differences were found