Fig. 5

Phagocytic activity. Phagocytic activity of MucilAir™ cell monolayer treated with undiluted Rhinosectan^® after 15 and 60 min. 0.9% NaCl saline solution was used as a negative control. Latex Beads-Rabbit IgG-FITC solution was added to the cell monolayer at a 1:10 dilution simultaneously with 30 μl of Rhinosectan^® or saline solution and incubated at 37 °C for 15 or 60 min. Cells were washed with assay buffer and a visualized at ×10 magnification with the inverted fluorescence microscope or b the fluorescence intensity of the cells was measured in a fluorescence plate reader with the appropriate filter sets (ex/em 485/535 nm). a Duplicate tissues have been used for fluorescence evaluation but only a selection of images is reported. The first two images correspond to saline solution at baseline, the second ones to Rhinosectan at 15 min post-treatment and third ones to Rhinosectan at 60 min post-treatment. b The average of two independent experiments and standard deviation bars are shown. Asterisk show p-values lower than 0.05 (Student T test)