Fig. 4

Frequency of TNFα producing cells (a) and relative amount of TNFα produced per cell (MFI) (b) among CD16⁺ monocytes, following in vitro stimulation with Dermatophagoides pteronyssinus (activated state) or without stimulation (basal state), in the control group, non-SIT group and SCIT group, immediately before treatment (T0) or 4 h after (T4). Mann–Whitney U test was used to compare control, non-SIT and SCIT-T0/T4 groups. Student’s t test was used to compare SCIT-T0 versus the SCIT-T4 group. The results were given by median with interquartile range. Statistical significant differences were considered when p < 0.05; *between the groups indicated in the figure