Skip to main content

Table 1 Antibodies used for immunohistochemical analyses

From: Structural alterations and markers of endothelial activation in pulmonary and bronchial arteries in fatal asthma

Antibody

Pre-treatment

Dilution autopsy tissue

Clone

Manufacturer

Collagen I

Citrate

1:2500

Polyclonal

US Biological, Swampscott, MA, USA

Collagen III

Pepsin

1:15

 

Abcam, Cambridge, USA

Fibronectin

Citrate

1:12000

Polyclonal

Dako, Glostrup, Denmark

Tenascin

Pepsin

1:20000

BC-24

Sigma-Aldrich, Missouri, USA

Versican

Trypsin

1:1000

2-b-1

Seikagaku Co. Tokyo, Japan

AT2

Citrate

1:1000

EPR3876

Abcam, Cambridge, USA

Endothelin-1

Citrate

1:300

 

Santa Cruz Biotechnology, CA, USA

ICAM-1

Tris–EDTA

1:50

23G12

Abcam, Cambridge, USA

VCAM-1

Citrate

1:100

 

Santa Cruz Biotechnology

  1. AT2 angiotensin II type 2 receptor, ET-1 endothelin-1, ICAM-1 intercellular adhesion molecule 1, VCAM-1 vascular cell adhesion molecule 1