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Fig. 1 | Allergy, Asthma & Clinical Immunology

Fig. 1

From: Differential expression of CCR2 and CX3CR1 on CD16+ monocyte subsets is associated with asthma severity

Fig. 1

Gating strategy for defining monocyte subsets. a A side scatter (SSC) versus forward side scatter (FSC) dot plot was used to identify cells from debris (P1); b a SSC/CD45 dot plot from live gate (P2) was used to gate on CD45+ cells and exclude large granulocytes; c a FSC/SSC dot plot from the CD45+ expressing cells was used to tightly gate on the monocyte population (positioned just above the lymphocytes); d a CD14/CD16 dot plot from CD14+ monocytes was arbitrarily set based on their fluorescent intensity and on FMO and isotype controls to reveal the three monocyte subsets (CD14++CD16, CD14++CD16+, and CD14+CD16++); e the expression of CCR2 and CX3CR1 by the three monocyte subsets was set based on FMO and isotype control

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