Fig. 1

Gating strategy for defining monocyte subsets. a A side scatter (SSC) versus forward side scatter (FSC) dot plot was used to identify cells from debris (P1); b a SSC/CD45 dot plot from live gate (P2) was used to gate on CD45⁺ cells and exclude large granulocytes; c a FSC/SSC dot plot from the CD45⁺ expressing cells was used to tightly gate on the monocyte population (positioned just above the lymphocytes); d a CD14/CD16 dot plot from CD14⁺ monocytes was arbitrarily set based on their fluorescent intensity and on FMO and isotype controls to reveal the three monocyte subsets (CD14⁺⁺CD16⁻, CD14⁺⁺CD16^+, and CD14⁺CD16⁺⁺); e the expression of CCR2 and CX₃CR1 by the three monocyte subsets was set based on FMO and isotype control