Fig. 4

Identification and purification of hyaluronidase from V. affinis venom and comparison of its activity with hyaluronidase of A. dorsata venom: a venom profile obtained from high performance liquid chromatography (HPLC); F1 to F3—SDS-PAGE of the fractions eluted, CV—SDS-PAGE of crude V. affinis venom, F4—SDS-PAGE of hyaluronidase fraction of V. affinis venom and F4 IB—IgE reactivity to eluted F4 fraction in immunoblot and b comparison of hyaluronidase enzyme activity in V. affinis venom, A. dorsata venom and hyaluronidase fractions eluted from V. affinis and A. dorsata venom