Fig. 2

Regulatory effect of IL-35 on the proliferation of HMC-1 cells. a HMC-1 cells were treated with IL-35 (1–1000 ng/ml) for 6 h. Cell viability was measured by CCK8. b HMC-1 cells were treated with IL-35 (1–100 ng/ml) for 6 h and stimulated with PMA (50 nM) plus A23187 (1 μM) for 8 h. Cell viability was measured by CCK8. All data are expressed as mean ± SD. P values are based on the One-way analysis of variance. n = 10