Fig. 5From: Interleukin-35 inhibited the production of histamine and pro-inflammatory cytokines through suppression MAPKs pathway in HMC-1 cellsEffect of IL-35 on PMA plus A23187-stimulated MAPKs activation. a After pretreatment of IL-35 for 6 h, HMC-1 cells were stimulated by PMA (50 nM) plus A23187 (1 μM) for 15 min for MAPKs activation. Phosphorylation of ERK1/2, JNK1/2, and p38 MAPKs was analyzed by western blotting. b The intensity was measured by densitometry. All data are expressed as mean ± SD. P values are based on the One-way analysis of variance. n = 10. ****P < 0.0001 compared with PMA plus A23187 groupBack to article page