Table 1 qRT-PCR primer pairs
From: MiRNA-221-5p suppressed the Th17/Treg ratio in asthma via RORγt/Foxp3 by targeting SOCS1
Name | Forward | Reverse |
|---|---|---|
IL-6 | 5'-ACAACCACGGCCTTCCCTACT-3' | 5'-CTCATTTCCACGATTTCCCAGA-3' |
IL-17 | 5'-GCTGTTGCTGCTGCTGAG-3' | 5'-TGGAACGGTTGAGGTAGTC-3' |
IL-21 | 5'-GGACCCTTGTCTGTCTGGTAG-3' | 5'-TGTGGAGCTGATAGAAGTTCAGG-3' |
IL-22 | 5'-ATGAGTTTTCCCTTATGGGGAC-3' | 5'-GCTGGAAGTTGGACACCTCAA-3' |
IL-10 | 5'-GGACAACATACTGCTAACCGACTC-3' | 5'-TTCATGGCCTTGTAGACACCTT-3' |
IL-35 | 5'-TATGGTCAGCGTTCCAACAGC-3' | 5'-TTCGGGACTGGCTAAGACACC-3' |
TGF-β | 5'-CAAACTAAGGCTCGCCAGTCC-3' | 5'-TTGCGGTCCACCATTAGCAC-3' |
β-actin | 5'-GTGACGTTGACATCCGTAAAGA-3' | 5'-GTAACAGTCCGCCTAGAACAC-3' |
U6 | 5'-GCTTCGGCAGCACATATACTAAAAT-3' | 5'-CGCTTCAGAATTTGCGTGTCAT-3' |