Fig. 1

Experimental timeline of mouse model establishment and intervention. Forty female BALB/c mice were randomly divided into four groups (n = 10/group). The abdomens and backs of the mice were exposed to 20 μL of 7% 2,4-dinitrochlorobenzene (DNCB) for 2 consecutive days, and the back skin was additionally challenged with 40 μL of 1% DNCB and 40 μL of ovalbumin (OVA)-PBS solution every 3 days, for a total of four rounds. The control group was treated in the same manner with acetone solution. On day 21, the mice were challenged via inhalation of aerosolized 1% OVA solution for 30 min every day for 7 consecutive days. Intraperitoneal injection of AS1517499 (10 mg/kg; dissolved in corn oil) and corn oil were administered to the AD and control groups, respectively, 1 h before aerosol inhalation (days 21, 23, 25, and 27). On day 30, the mice were sacrificed, and the required samples were collected