Fig. 5

KIF2A mediated autophagy induction and IL-33 secretion in epithelial cells. A MLE-12 cells were transfected with pCMV6-KIF2A or vehicle and then exposed to HDM (50 μg/mL) 36 h after transfection. 24 hous after HDM exposure, the expression of KIF2A was examined using qPCR. B MLE-12 cells were left untreated or treated with HDM as described in (A). The TSLP, IL-25, and IL-33 expression levels were examined using qPCR. C Representative immunofluorescence images showing autophagy levels in different groups. Scale bar, 5 μm. D MLE-12 cells were transfected with pCMV6-KIF2A or vehicle and then exposed to HDM (50 μg/mL) 36 hous after transfection. The expression levels of KIF2A, autophagy, and IL-33 were examined 24 hous after HDM exposure with Western blotting and the density quantification was measured as a ratio relative to β-actin. Data are presented as mean ± SEM of independent experiments with similar results (n = 4 ~ 6). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001