Fig. 5

MiR-224-5p reduces airway inflammation in asthmatic mice through FHL1 suppression. Asthmatic mice were treated with lentivirus harboring miR-224-5p-agomir/NC-agomir alone or in combination with lentivirus harboring oe-FHL1/oe-NC. A Expression of miR-224-5p and FHL1 in lung tissues of asthmatic mice determined by RT-qPCR. B The airway resistance in asthmatic mice on the 1st day. C The airway resistance in asthmatic mice at various time points when the dosage of Mch is 200 mg/mL. D, The airway responsiveness in asthmatic mice. E The number of total cells (the left) and percentage of eosinophils in BALF of asthmatic mice. F Levels of IL-4, IL-5, IL-13 and IFN-γ in BALF supernatant of asthmatic mice measured by ELISA. G HE staining images of lung tissues from asthmatic mice. H Masson’s trichrome staining images of lung tissues and collagen deposition area from asthmatic mice. * p < 0.05 compared with asthmatic mice treated with lentivirus harboring NC-agomir. # p < 0.05 compared with asthmatic mice treated with lentiviruses expressing miR-224-5p-agomir and oe-NC. n = 10 mice for each treatment