Fig. 1
Experimental schedule for intraperitoneal sensitization, immunotherapy, and intratracheal challenge. BALB/c mice were IP-sensitized with 5 µg nDer p 2 which emulsified with Al(OH)3 on days 1, 7, and 14 except for the Naïve group. Immunotherapy was conducted continuously on days from 21 to 42. The mice were fed according to the following group arrangement. Naïve: the entire experiment was done without any manipulation used as a reference for physiological state and environmental variables. Saline: mice fed with saline 100 µl/ mouse/ day after sensitization. 1217A: fed with 2.5 mg/ 00 µl/mouse/ day modified LWDHW-1217A after sensitization. 1217B: fed with 2.5 mg/100 µl/mouse/day modified LWDHW-1217B. DEX: fed with Dexamethasone 1 µg/100 µl/mouse/day. AHR: Airway hyperresponsiveness; Sac: Sacrifice after the AHR measurement on day 46