Fig. 6
Effects of 1217A and 1217B on the Gene expressions of the inflammatory cytokines, chemokine, and transcription factors in lung tissues. Total mRNA was extracted from the lung tissue of mice after sacrifice. The mRNA of lung tissue was acquired for real-time quantitative-PCR assay (Q-PCR) to investigate gene expressions. The TH2-related genes (IL-4, IL-5, and IL-13), TH2-related transcription factor (GATA-3), neutrophil chemotactic chemokine (IL-8), and the TH1-related transcription factor (T-bet) were used to evaluate the gene variations. GAPDH is used as an internal control gene for normalizing the target gene expression of each sample. The histogram shows the normalized expression levels of the target gene/ GAPDH as mean ± SD. #: p < 0.05 compared to the Naive group; *: p < 0.05 compared to the treatment with saline