Fig. 9

Differences in airway hyperresponsiveness (AHR) to methacholine were measured among these groups after immunotherapy. Pulmonary function of mice was measured after intratracheal challenge with allergen-nDer p 2 in the Naïve group and immunotherapy groups. Methacholine plays the role of a bronchoconstriction agent at a concentration of 6.25, 12.5, and 25 mg/ml. PBS Inhalation was the baseline of pulmonary function. The AHR to methacholine was measured 30 min after the intratracheal challenge, and the AHR presented as enhanced Pause (Penh) values with means ± SD. #: p < 0.05 compared to the Naïve group; *: p < 0.05 compared to the saline group