Volume 10 Supplement 1
Migration of the contractile phenotype of human airway smooth muscle cells in response to supernatants from rhinovirus infected human bronchial epithelial cells
© Qureshi et al; licensee BioMed Central Ltd. 2014
Published: 3 March 2014
Human rhinovirus (HRV) infections during early childhood are associated with a significantly increased risk of developing asthma in subsequent years . There is published evidence that airway remodeling is present in pre-school children, often before the diagnosis of asthma is established . It is thought that this increased risk relates to the fact that HRV infections facilitate airway remodeling in asthma . A feature of airway remodeling is the proximity of airway smooth muscle (ASM) to the subepithelial region among other pathological changes . Smooth muscle is also known to exist in two distinct phenotypes: secretory and contractile . We have recently shown that HRV infection of Human Bronchial Epithelial Cells (HBEC), both in vitro and in vivo, results in the up-regulation of a number of airway remodeling mediators . We now sought to determine which ASM phenotype (contractile or secretory) results in migration to supernatants from HRV infected HBE cells.
Primary HBE cells were cultured in growth medium until confluent, pre-treated with 1% insulin, transferrin, and selenium (ITS) medium for 24 hours and then stimulated with media-control or purified HRV-16. The ASM D9 cell-line was obtained from Dr. Andrew Halayko’s laboratory and cultured in T-175 flasks in 10% serum containing Dulbecco's Modified Eagle Medium (DMEM; Gibco; secretory phenotype) or 1% ITS F-12 media (Gibco; contractile) until they reach ~90-100% confluence. HBEC supernatants were used as chemo-attractants for ASM (4hrs) migration through 8 μm pore polycarbonate filter in a 48-well Boyden Chamber. Migrated cells on filter were fixed/stained via Diff-quick and counted at 200x view.
ASM D9 cells treated with 1% ITS F12 media showed significantly higher levels of migration to fetal bovine serum (FBS) compared to ASM D9 cells treated with serum (n=3, p < 0.001). Preliminary data indicate that HRV-16 infected HBEC supernatants resulted in greater ASM migration compared to HBEC supernatant from media alone in the ASM D9 contractile phenotype but not the secretory phenotype.
These findings support our hypothesis that the contractile phenotype of ASM D9 cells, which is more representative of ASM cells in vivo, migrate better than the secretory phenotype. Additionally, strong preliminary data indicate that supernatants from HRV infected HBE cells promotes ASM chemotaxis, and provides additional evidence for the role of contractile ASM, and HRV infections, in the pathogenesis of airway remodeling. Ongoing studies will examine protein expression between the two different phenotypes.
This abstract was funded in part thanks to the AllerGen NCE., and the GSK-CIHR Professorship in inflammatory lung disease.
- Lemanske RF: The childhood origins of asthma (COAST) study. Pediatr Allergy Immunol. 2002, 13: 38-43.View ArticlePubMedGoogle Scholar
- Proud D, Leigh R: Epithelial cells and airway diseases. Immunological reviews. 2011, 242: 186-204.View ArticlePubMedGoogle Scholar
- Gerthoffer WT: Migration of airway smooth muscle cells. Proc Am Thorac Soc. 2008, 5: 97-105.PubMed CentralView ArticlePubMedGoogle Scholar
- Sharma P: Expression of the dystrophin-glycoprotein complex is a marker for human airway smooth muscle phenotype maturation. Am J Physiol Lung Cell Mol Physiol. 2008, 294: L57-L68.View ArticlePubMedGoogle Scholar
- Leigh R: Human rhinovirus infection enhances airway epithelial cell production of growth factors involved in airway remodeling. J Allergy Clin Immunol. 2008, 121: 1238-1245.e4.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.