Volume 10 Supplement 1
Mapping of novel chromosomal regions associated with atopy
© Kanagaratham et al; licensee BioMed Central Ltd. 2014
Published: 3 March 2014
Naïve mice from each RCS were phenotyped for atopy by measuring plasma IgE concentration by ELISA. RCS mice were genotyped at 1215 markers that spanned the entire genome. Using the log transformed phenotype values and genotyping data, a marker-by-marker association analysis was performed to identify associations between the strain phenotype and genotype, while correcting for major background strain. Within the phenotype associated loci, candidate genes were selected based on the presence of coding mutations between the sequences of the two parental strains.
To the best of our knowledge, we have identified a novel candidate loci associated with atopy. Future plans of our study include functionally validating the importance of our candidate genes, candidate locus, and of chromosome 3 in atopy. Our results demonstrate that using a genetically unique panel of RCS we can identify candidate genes that are in common and unique to the various phenotypes of allergic asthmatics.
- Fortin A, Diez E, Rochefort D, Laroche L, Malo D, Rouleau GA: Recombinant congenic strains derived from A/J and C57BL/6J: a tool for genetic dissection of complex traits. Genomics. 2001, 74: 21-35.View ArticlePubMedGoogle Scholar
- Camateros P, Marino R, Fortin A, Martin JG, Skamene E, Sladek R: Identification of novel chromosomal regions associated with airway hyperresponsiveness in recombinant congenic strains of mice. Mamm Genome. 2010, 21: 28-38.View ArticlePubMedGoogle Scholar
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