Figure 1

Segmental allergen challenge (SAC) induces an inflammatory response detected in the BAL fluid of atopic asthmatics. In A), total cell counts and differential analysis are presented for BAL fluid obtained pre- and post-SAC. In B), cytokine levels in the BAL fluid were measured by multiplex ELISA using the Cytokine Bead Array (CBA). Shown is the re-analysis performed on the 24 subjects enrolled in the randomized, placebo controlled, double blinded Costimulation Inhibition of Asthma (CIA) trial at pre-randomization. In A (right panel), the average (change in percent cell type) is presented with error bars representing ± the standard deviation with an (* = p < 0.05; ** = p <0.01; *** = p < 0.001). Each subject is represented by a single circle in the graphs with p values presented. Due to pre-SAC cytokines being below the detection limit of the assay (5 pg/mL), no statistical analysis was performed for IL-10, IL-2 or IFN-γ.