Figure 2

Innate and adaptive immune cells are recruited to the BAL fluid after allergen challenge. Cellular populations in the BAL fluid pre- and post-SAC were labeled with specific fluorophore conjugated antibodies and analyzed by multi-color flow cytometry. The percentages were calculated as a percent of the lymphocyte gate, with the exception of the DC (plasmacytoid [pDC], myeloid [mDC]) and myeloid derived suppressor cell (MDSC) subsets which were calculated as a percentage of the lineage cocktail negative gate. Each subject is represented by a single symbol on the graphs, with p values indicated.