Figure 4

CD30 expression is detected after allergen challenge in the BAL fluid. In A), eosinophils were analyzed for CD30 expression by flow cytometry by gating on high side scatter (left panel) followed by gating on CD16 negative cells with eosinophils determined as CD11b+. Shown are flow cytometry plots for a representative subject with CD30 expression on eosinophils compared to an isotype control (A, right 2 panels). In B) (left panel), membrane CD30 expression was analyzed on lymphocyte populations (CD4+ and CD8+ T cells ● and ○; B cells □ and Tregs ◊) post-SAC on 7 subjects by multi-color flow cytometry, with p-values determined by 1-way ANOVA (Kruskal-Wallis) with Dunn’s Multiple Comparison Test for individual means indicated. In B) (right panel), sCD30 was measured in the pre- and post-SAC BAL of 10 subjects by ELISA, with the p-value indicated.